Summary
Type: TLC
Application #: 401960
Catalog#: 2414056 - Nano-Silica w/Concentration Zone HPTLC Plates, Glass Backed, 200um, 10x10cm, 25pk
Detection: TLC-scanner at 355 nm or photographing
Plates are dipped 5 sec. into a solution of 10% CuSO4 * 5 H2O (w/v) in 8% H3PO4 (v/v), than placed vertically on paper towels for 30 sec. to drain excessive cha
Conditions: Nano-SILGUR-20 predeveloped with methanol and eluent 1, activated at 110 °C for 10 min.
Eluent 1: methyl acetate:1-propanol:chloroform:methanol:0.25% KCl (25:25:25:10:9)
Eluent 2: n-hexane:diethyl ether:acetic acid (75:23:2)
Eluent 3: n-hexane
Migration distance:
1. development: eluent 1, 4.5 cm, 13 min.
2. development: eluent 2, 7 cm, 12 min.
3. development: eluent 3, 7 cm, 12 min.
After each development the plates were thoroughly dried
Sample volume: 1-25 μl
Authors: Klein TR
Source: Biol Chem 379 (1998) 655-666